Journal: Journal of biomedical materials research. Part A

Article Title: Vitronectin is A Critical Protein Adhesion Substrate for IL-4-INDUCED Foreign Body Giant Cell Formation

doi: 10.1002/jbm.a.31658

Figure Lengend Snippet: Monocyte/macrophage adhesion, morphology, and FBGC formation on RGD-modified polystyrene. RGD peptide was adsorbed at 25 μg per ml. Monocytes were plated and cultured for 1.5 hr (day 0), or until days 3 or 7. FBGC formation was induced with IL-4 on day 3. May-Grünwald/Giemsa. 20X

Article Snippet: On day 3, the monocytes/macrophages were either fixed with methanol or the medium was replaced with 0.2 ml of SFM with 5% heat-treated autologous serum (1 hr at 56°C) and 15 ng/ml recombinant human IL-4 (R&D Systems, Minneapolis, MN).

Techniques: Modification, Cell Culture

Journal: Journal of biomedical materials research. Part A

Article Title: Vitronectin is A Critical Protein Adhesion Substrate for IL-4-INDUCED Foreign Body Giant Cell Formation

doi: 10.1002/jbm.a.31658

Figure Lengend Snippet: Adhesion to protein- or RGD-adsorbed polystyrene. Proteins or RGD peptide were adsorbed at a concentration of 25 μg per ml. Following removal of unadsorbed proteins by washing, monocytes were plated and cultured for 1.5 hr (day 0), or until days 3 or 7 and stained with May-Grünwald/Giemsa. FBGC formation was induced with IL-4 on day 3. Adhesion was determined as described in Methods. Results represent mean adherent cell number ± SEM, n = 3 different monocyte donors. *Significantly different from other adsorbed proteins (P<0.05).

Article Snippet: On day 3, the monocytes/macrophages were either fixed with methanol or the medium was replaced with 0.2 ml of SFM with 5% heat-treated autologous serum (1 hr at 56°C) and 15 ng/ml recombinant human IL-4 (R&D Systems, Minneapolis, MN).

Techniques: Concentration Assay, Cell Culture, Staining

Journal: Journal of biomedical materials research. Part A

Article Title: Vitronectin is A Critical Protein Adhesion Substrate for IL-4-INDUCED Foreign Body Giant Cell Formation

doi: 10.1002/jbm.a.31658

Figure Lengend Snippet: Monocyte-to-macrophage development and FBGC formation on adsorbed proteins. CN I, C3bi, FG, FN, or VN were adsorbed at a concentration of 25 μg per ml. Monocytes were plated and cultured for 1.5 hr (day 0), or until days 3 or 7, and stained with May-Grünwald/Giemsa. FBGC formation was induced with IL-4 on day 3. 20X

Article Snippet: On day 3, the monocytes/macrophages were either fixed with methanol or the medium was replaced with 0.2 ml of SFM with 5% heat-treated autologous serum (1 hr at 56°C) and 15 ng/ml recombinant human IL-4 (R&D Systems, Minneapolis, MN).

Techniques: Concentration Assay, Cell Culture, Staining

Journal: Journal of biomedical materials research. Part A

Article Title: Vitronectin is A Critical Protein Adhesion Substrate for IL-4-INDUCED Foreign Body Giant Cell Formation

doi: 10.1002/jbm.a.31658

Figure Lengend Snippet: Macrophage fusion protein- or RGD-adsorbed polystyrene. Proteins or RGD peptide were adsorbed at a concentration of 25 μg per ml. Monocytes were plated and cultured for 7 days as described in Methods with the IL-4-induction of macrophage fusion on day 3. Following May-Grünwald/Giemsa staining, percent macrophage fusion was determined. Results represent mean % fusion ± SEM, n = 3 different monocyte donors. *Significantly different from other adsorbed proteins (P<0.05).

Article Snippet: On day 3, the monocytes/macrophages were either fixed with methanol or the medium was replaced with 0.2 ml of SFM with 5% heat-treated autologous serum (1 hr at 56°C) and 15 ng/ml recombinant human IL-4 (R&D Systems, Minneapolis, MN).

Techniques: Concentration Assay, Cell Culture, Staining

Journal: Journal of biomedical materials research. Part A

Article Title: Vitronectin is A Critical Protein Adhesion Substrate for IL-4-INDUCED Foreign Body Giant Cell Formation

doi: 10.1002/jbm.a.31658

Figure Lengend Snippet: VN-supported IL-4-induced FBGC formation: Concentration dependence. VN was not adsorbed or adsorbed at 2.5, 5, or 25 μg per ml, and monocytes were plated and cultured for 7 days as described in Methods with the IL-4-induction of macrophage fusion on day 3. May-Grünwald/Giemsa. 20X

Article Snippet: On day 3, the monocytes/macrophages were either fixed with methanol or the medium was replaced with 0.2 ml of SFM with 5% heat-treated autologous serum (1 hr at 56°C) and 15 ng/ml recombinant human IL-4 (R&D Systems, Minneapolis, MN).

Techniques: Concentration Assay, Cell Culture

Journal: Journal of biomedical materials research. Part A

Article Title: Vitronectin is A Critical Protein Adhesion Substrate for IL-4-INDUCED Foreign Body Giant Cell Formation

doi: 10.1002/jbm.a.31658

Figure Lengend Snippet: VN-supported IL-4-induced macrophage fusion: Concentration dependence. VN was not adsorbed or adsorbed at the indicated concentrations, and monocytes were plated and cultured for 7 days as described in Methods with the IL-4-induction of macrophage fusion on day 3. Following May-Grünwald/Giemsa staining, percent macrophage fusion was determined. Results represent mean % fusion ± SEM, n = 3 different monocyte donors.

Article Snippet: On day 3, the monocytes/macrophages were either fixed with methanol or the medium was replaced with 0.2 ml of SFM with 5% heat-treated autologous serum (1 hr at 56°C) and 15 ng/ml recombinant human IL-4 (R&D Systems, Minneapolis, MN).

Techniques: Concentration Assay, Cell Culture, Staining